Neurovirulence of Type 1 Polioviruses Isolated from Sewage in Japan

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Neurovirulence of type 1 polioviruses isolated from sewage in Japan.

Sixteen type 1 poliovirus strains were isolated from a sewage disposal plant located downstream of the Oyabe River in Japan between October 1993 and September 1995. The isolates were intratypically differentiated as vaccine-derived strains. Neutralizing antigenicity analysis with monoclonal antibodies and estimation of neurovirulence by mutant analysis by PCR and restriction enzyme cleavage (MA...

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Characterisation of vaccine-derived polioviruses isolated from sewage and river water in Japan.

BACKGROUND A nucleotide change from U to C at position 472 in the 5' non-coding region of the type 3 poliovirus is associated with increased neurovirulence. Moreover, the proportion of type 3 polioviruses containing this mutation (472-C revertants) correlates with the neurovirulence of a particular sample. We used mutant analysis by PCR and restriction-enzyme cleavage (MAPREC) to estimate the n...

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Seventy-eight poliovirus strains isolated from river water and sewage in Toyama Prefecture, Japan, during 1993 to 1995 were characterized by the PCR-restriction fragment length polymorphism (RFLP) method and by partially sequencing the VP3 and VP1 regions of the viral genome. Of these isolates, 36 were identified as Sabin vaccine strains, and 42 were identified as vaccine variant strains that h...

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Neurovirulent Vaccine-Derived Polioviruses in Sewage from Highly Immune Populations

BACKGROUND Vaccine-derived polioviruses (VDPVs) have caused poliomyelitis outbreaks in communities with sub-optimal vaccination. Israeli environmental surveillance of sewage from populations with high (>95%) documented vaccine coverage of confirmed efficacy identified two separate evolutionary clusters of VDPVs: Group 1 (1998-2005, one system, population 1.6x10(6)) and Group 2 (2006, 2 systems,...

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Newcastle disease (ND) is one of the contagious viral diseases in avian species. Recently, several ND outbreaks in pigeon caused by pigeon paramyxovirus serotype-1 (PPMV-1) have been reported in limited numbers from Iran and phylogenetic studies have been conducted on partial sequence of NDV fusion (F) gene. In the present study, ten PPMV-1, named Pigeon_paramyxovirus1_isolate_pigeon/Iran/UT_EG...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2002

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.68.1.138-142.2002